CERTAIN DERIVATIVES OF THE PROTEIDS. 313 



at 100 C. in a large steam sterilizer (Arnold's), with occasional 

 agitation, for 12 to 15 hours, after which the gelatinous anti- 

 albumid was separated from the acid solution (containing the 

 hemi bodies) by filtration through paper and freed from all 

 soluble matter by long continued washing with water. The 

 washing was continued until the wash-water reacted only 

 faintly acid. In some cases the antialbumid was again heated 

 at 100 C. for 12 hours with a fresh lot of 4 per cent sulphuric 

 acid. The crude antialbumid was next warmed at 40 C. for 

 forty hours with a large volume of an active solution of 

 pepsin-hydrochloric acid for the complete removal of adherent 

 hemi bodies or of any unaltered egg-albumin. The residual 

 antialbumid was then filtered off and washed thoroughly with 

 distilled water until the washings were free from chlorine. 

 The product was next dissolved in a solution of 0.5 per cent 

 sodium carbonate, the fluid filtered through paper, and the 

 antialbumid reprecipitated by neutralization with 0.2 per cent 

 hydrochloric acid, after which it was again washed with water 

 until free from chlorine, and lastly with 95 per cent alcohol. 

 This product constitutes antialbumid (A). 



In separating the so-called hemialbumoses from the 4 per 

 cent sulphuric acid solution, the clear filtered fluid was neu- 

 tralized with ammonia and evaporated to a convenient volume, 

 the neutralization precipitate filtered off, and the further con- 

 centrated fluid eventually saturated boiling hot with ammonium 

 sulphate in acid, neutral and alkaline reaction according to the 

 method suggested by Kiihne.* The precipitate of mixed 

 albumoses was dissolved in water, a little thymol added, and 

 the fluid dialyzed in running water until the sulphate was 

 entirely removed. When the dialysis was completed a small 

 quantity of heteroalbumose was found adherent to the parch- 

 ment. This was therefore filtered off, washed thoroughly with 

 water, lastly with alcohol, and dried. This product constitutes 

 heteroalbumose (-H"). Assuming the correctness of Neumei- 

 ster's statement that heteroalbumose as formed in pepsin- 

 proteolysis is composed mainly of anti groups, it suggests that 

 * Kiihne, Zeitschr. f . Biologic, 1892, xxix, p. 1. 



