314 A CHEMICO-PHYSIOLOGICAL STUDY OF 



the formation of this substance in the hydrolysis with sulphuric 

 acid may be taken as a measure of the extent to which in this 

 hydrolysis anti groups are split off in forms other than antial- 

 bumid. On the other hand, there is perhaps no logical reason 

 why a heteroalbumose might not be formed under this peculiar 

 method of hydrolysis, out of hemi groups mainly. 



The above fluid freed by dialysis from ammonium sulphate 

 and heteroalbumose, after filtration from the latter substance, 

 was concentrated to a syrup, precipitated with alcohol, and 

 the precipitate washed thoroughly with hot alcohol and dried. 

 This product constitutes hemialbumoses ( 6r), and is composed 

 of a mixture of hemiprotoalbumose and hemideuteroalbumose. 



The hemipeptone contained in the ammonium sulphate- 

 saturated fluid after separation of the mixed albumoses was 

 obtained as follows. The fluid was concentrated somewhat, 

 cooled, and the crystals of ammonium sulphate separated, this 

 operation being repeated four times, thus accomplishing the 

 removal of a large amount of the contained sulphate. During 

 the repeated heating of this solution a small quantity of a 

 dark oily albumose-like substance was obtained, which was 

 filtered off and freed from ammonium sulphate by the method 

 used in the purification of the peptone, i. e., with barium car- 

 bonate, etc. The product so isolated constitutes the albumose- 

 like body (M\ The solution was next treated with barium 

 hydroxide until the larger portion of the sulphate was precip- 

 itated, after which the last portions of the ammonium salt 

 were removed by warming the solution with pure barium car- 

 bonate. The filtered fluid was then treated with very dilute 

 sulphuric acid, drop by drop, until the last trace of dissolved 

 barium was removed and the filtrate concentrated to a small 

 volume, precipitated with alcohol, and the precipitate washed 

 repeatedly with boiling alcohol. This product constitutes 

 hemipeptone (I). 



Formation of antialbumoses by the action of pepsin-acid on 

 pure antiaibumid. When antialbumid is warmed at 40 C. 

 with an active solution of pepsin-hydrochloric acid (0.2 per 

 cent HC1) there results a slow but gradual digestion of the 



