CERTAIN DERIVATIVES OF THE PROTEIDS. 315 



proteid with formation of soluble antialbumoses together with 

 a trace of peptone. Using antialbumid (A) as the mother 

 substance, digestion with pepsin-acid was carried on for some 

 days, the undigested residue filtered off, the solution neutral- 

 ized with sodium carbonate, concentrated, and the albumoses 

 separated by saturation of the boiling solution with ammonium 

 sulphate. The gummy precipitate so obtained was dissolved 

 in water, dialyzed in running water until free from sulphate, 

 then concentrated to a syrup and precipitated with alcohol. 

 The product was a mixture of protoalbumose and deuteroalbu- 

 mose, and constitutes the preparation called antialbumoses () 

 Antialbumoses and antipeptone formed by the action of an 

 alkaline solution of trypsin on antialbumid. Antialbumid (A) 

 was dissolved in 0.5 per cent sodium carbonate and the solu- 

 tion warmed at 40 C. for five days with a purified solution 

 of trypsin,* also in 0.5 per cent sodium carbonate, thymol 

 being added to prevent putrefaction. After the clear solution 

 had been at 40 C. for a few hours it became opaque and 

 soon formed a thick jelly due to the separation of a portion of 

 the antialbumid in a slightly modified form, as has frequently 

 been described in other places.f At the expiration of the 

 five days a large proportion of this insoluble matter had dis- 

 appeared, but there still remained an appreciable amount of 

 the substance, which was filtered off, thoroughly washed with 

 water, and dried. This constitutes antialbumid (-&) The 

 alkaline solution containing the albumoses and peptone was 

 neutralized with dilute hydrochloric acid, the slight precipitate 

 removed by filtration, and the fluid concentrated to a con- 

 venient volume. The antialbumoses and antipeptone were 

 then separated by ammonium sulphate and isolated exactly as 



* The trypsin solution was prepared by warming 40 grams of Kiihne's dry 

 ox pancreas in 400 c.c. 0.1 per cent salicylic acid solution for 24 hours, mak- 

 ing the extract alkaline (0.25 per cent Na 2 CO 8 ) and warming this at 40 C. 

 for 48 hours in the presence of thymol. The extract was next dialyzed for 

 some days from both alkaline and acid (acetic acid) solution, then evaporated 

 to dryness at 40 C. and the residue taken up with a very little cold water 

 This solution, filtered, contains fairly pure trypsin. 



t Kiihne and Chittenden, Zeitschr. f. Biologic, 1883, xix, p. 166. 



