316 A CHEMICO-PHYSIOLOGICAL STUDY OF 



described under the head of hemialbumoses and hemipeptone. 

 The antipeptone, however, owing to the presence of sodium 

 chloride, was further purified by dialysis until the chloride 

 was entirely removed. The product is antipeptone (D). 



The antialbumoses, indicated as antialbumoses ((7), were a 

 mixture of proto and deuteroalbumose. This is a matter of 

 some moment chemically, for it is generally assumed that in 

 trypsin-proteolysis primary proteoses are not formed; that 

 the proteid passes directly to deuteroproteose and thence 

 into true peptone, etc.* Further, it is implied in the scheme 

 of pepsin-proteolysis generally accepted by writers on this 

 subject, that antialbumid undergoing gastric digestion is 

 transformed directly into antideuteroalbumose without any 

 intermediate formation of a primary proteose.f This, how- 

 ever, is not the case ; certainly not where pure antialbumid is 

 undergoing digestion. Thus, we have observed that in the 

 digestion of antialbumid with both gastric juice and pancreatic 

 juice, the albumoses formed and separated contained a large 

 amount of protoalbumose ; the neutral solution of the albu- 

 moses yielding a very decided precipitate on saturation with 

 sodium chloride. 



The antipeptone (D) formed by the action of trypsin on pure 

 antialbumid was found on analysis to have the following 

 composition, when dried at 110 C. 



I. 0.3003 gram substance gave 0.1812 gram H 2 = 6.70 per cent H and 



0.6502 gram CO 2 = 49.96 per cent C. 

 II. 0.3330 gram substance gave 0.6075 gram CO 2 = 49.75 per cent C. 



III. 0.2681 gram substance gave by the Kjeldahl method 0.03570 gram N = 



13.32 per cent N. 



IV. 0.2722 gram substance gave 0.03612 gram N = 13.27 per cent N. 



V. 1.0382 grams substance gave by fusion with NaOH t and KNO 8 0.1203 



gram BaSO 4 = 1.59 per cent S. 

 VI. 1.0665 grams substance gave 0.0228 gram ash = 2.14 per cent. 



* See Neumeister's Lehrbuch d. physiol. Chem., 1897, p. 247. 

 t See Neumeister, Zeitschr. f. Biologic, 1887, xxiii, p. 391. 

 t Pure sodium hydroxide made from the metal sodium and free from 

 sulphur. The fusion was made in a silver crucible over an alcohol lamp. 



