318 A CHEMICO-PHYSIOLOGICAL STUDY OF 



Another product to be referred to under the head of anti 

 bodies is antialbumid (.F). This substance was prepared from 

 well crystallized edestin,* a globulin obtained from hemp-seed, 

 by heating the substance at 100 C. with 4 per cent sulphuric 

 acid. The gelatinous antialbumid resulting by this process 

 was purified by solution in 0.5 per cent sodium carbonate, etc., 

 and then used for intravenous injection. 



Hydrolysis and cleavage of pure gelatin by trypsin in neutral 



solution In this hydrolysis 200 grams of exceptionally pure 



gelatin prepared from the purified collagen of white fibrous 

 connective tissue (tendons) by Dr. Van Name f were available. 

 This material was dissolved hi four liters of distilled water, 

 a neutral solution of purified trypsin J added, together with 

 sufficient thymol to prevent putrefaction, and the mixture 

 warmed at 40 C. for four weeks. The object of this long- 

 continued digestion was to insure the formation of as large an 

 amount as possible of true gelatin-peptone, the substance 

 usually described under this name being nothing more than a 

 mixture of gelatoses. Further, digestion was carried on in 

 a neutral fluid to obviate the necessity for removal from the 

 resultant products of alkali salts; a process which entails 

 great loss of substance. Careful examination of the digestive 

 mixture at the end of the four weeks showed the complete 

 absence of primary gelatoses. The solution was therefore 

 concentrated and the deuterogelatose separated directly by 

 saturation with ammonium sulphate under the conditions 

 already referred to. The product was purified by dialysis, 

 and eventually precipitated from a concentrated solution by 

 strong alcohol, after which it was repeatedly boiled with 

 alcohol for removal of any soluble extractives and then dried. 

 This product constitutes deuterogelatose (K.) The yield was 

 113 grams. 



Chittenden and Goodwin, Journal of Physiology, 1891, xii, p. 34; Balke, 

 Zeitschr. f. physiol. Chem., 1896, xxii, p. 248, etc. 



* Chittenden and Mendel, Journal of Physiology, 1894, xvii, p. 48. 



} Van Name, Journal of Experimental Medicine, 1897, ii, p. 117. 



} See foot-note (p. 315). 



