320 A CHEMICO-PHYSIOLOGICAL STUDY OF 



PERCENTAGE COMPOSITION OF THE ASH-FREE SUBSTANCE. 



I IL nL IV. V. Average. 



C 50.87 50.86 ... ... ... 60.87 



H 6.79 6.84 ... ... ... 6.81 



N ... ... 17.53 17.53 . . . 17.53 



S ... ... ... ... 0.34 0.34 



O ... ... ... ... ... 2445 



100.00 



ANALYSIS OF GELATIN-PEPTONE (L). 

 I. 0.2984 gram substance gave 0.1797 gram H 2 = 6.69 per cent H and 



0.5168 gram CO 2 = 47.23 per cent C. 



n. 0.2134 gram substance gave 0.1286 gram H 2 = 6.69 per cent H and 

 0.3709 gram CO 2 = 47.40 per cent C. 



III. 0.2869 gram substance gave by the Kjeldahl method 0.04827 [gram 



N = 16.82 per cent N. 



IV. 0.2168 gram substance gave 0.03661 gram N = 16.88 per cent N. 



V. 0.5017 gram substance gave by fusion with NaOH and KNO, 0.0119 gram 



BaSO 4 = 0.32 per cent S. 

 VI. 0.5376 gram substance gave 0.0114 gram ash = 2.12 per cent. 



PERCENTAGE COMPOSITION OF THE ASH-FBEE SUBSTANCE. 



L U. ILL IV. V. Average. 



C 48.25 48.42 ... ... ... 48.33 



H 6.83 6.83 ... ... ... 6.83 



N ... ... 17.18 17.24 . . . 17.21 



S ... ... 0.33 0.33 



... ... ... 27.30 



100.00 



Comparing these results with the composition of the pure 

 gelatin from which the gelatose and peptone were derived we 

 have the following figures : 



Gelatin.* Deuterogelatose. Gelatin-Peptone. 

 C 50.11 60.87 48.33 



H 6.66 6.81 6.83 



N 17.81 17.53 17.21 



S 0.26 0.34 0.33 



O 26.24 24.45 27.30 



/ 



Thus, again we have a forcible illustration of the fact that 

 in both pepsin- and trypsin-proteolysis the formation of true 

 peptone is associated with a marked diminution in the content 



* Van Name, loc. cit., p. 124. 



