CERTAIN DERIVATIVES OF THE PROTEIDS. 323 



will not yield tyrosin on treatment with trypsin, will yield con- 

 siderable tyrosin on decomposition with a strong mineral acid 

 at 100 C. So, likewise, these anti bodies may yield a large 

 proportion of the nitrogenous bases on decomposition with a 

 boiling mineral acid while wholly resistant to the action of 

 trypsin. 



In this connection it has seemed to us important to ascertain 

 how large a yield of nitrogenous bases can be obtained from 

 antialbumid by decomposition with a mineral acid, and also to 

 compare the results with those obtainable by a like decompo- 

 sition of a hemi body. For this purpose, 10 grams each of 

 antialbumid, hemialbumoses, and hemipeptone were boiled for 

 ninety-six hours with 40 c.c. of 20 per cent hydrochloric acid 

 with addition of a little stannous chloride. The solutions 

 were then diluted with water, the tin removed by hydrogen 

 sulphide, and the filtrates evaporated to syrupy consistency. 

 The residues were then dissolved in water and each made up 

 to 250 c.c. Measured portions of these solutions were used 

 for the determination of total nitrogen, nitrogen as ammonia, 

 and basic nitrogen precipitated by phosphotungstic acid, 

 following the methods used by E. Schulze.* Following are 

 the results obtained : 



ANTIALBUMID (A). 



Total nitrogen in the fluid 1.2745 grams. 



Nitrogen in phosphotungstic acid precipitate . . . 0.2782 " 



Nitrogen in the form of ammonia 0.0685 " 



Basic nitrogen, organic 0.2197 " 



17.2 per cent of total nitrogen in the form of bases precipitable by phospho- 

 tungstic acid. 



HEMIALBUMOSES (G). 



Total nitrogen in the fluid 1.3917 grams. 



Nitrogen in phosphotungstic acid precipitate . . . 0.4687 " 



Nitrogen in the form of ammonia 0.0805 " 



Basic nitrogen, organic 0.3882 " 



27.9 per cent of total nitrogen in the form of bases precipitable by phospho- 

 tungstic acid. 



* Schulze, E., Zeitschr. f. physiol. Chem., 1898, xxv, p. 360. 



