324 CERTAIN DERIVATIVES OF THE PROTEIDS. 



HEMIPEPTONE (I). 



Total nitrogen in the fluid 1.3770 grams. 



Nitrogen in phosphotungstic acid precipitate . . . 0.5492 " 



Nitrogen in the form of ammonia 0.2637 " 



Basic nitrogen, organic 0.2855 " 



20.7 per cent of total nitrogen in the form of bases precipitable by phospho- 

 tungstic acid. 



From these results we see that antialbumid differs only 

 quantitatively from the hemi bodies in yielding nitrogenous 

 organic bases on decomposition with a mineral acid; a fact 

 which affords additional evidence that the difference between 

 the so-called hemi and anti bodies is not due to the lack of 

 specific groups or radicles in the anti body, but rather to some 

 difference in the arrangement of the groups or the state of 

 combination by which the latter body is rendered resistant to 

 the enzyme trypsin. Particularly noteworthy is the large 

 proportion of nitrogen split off from hemipeptone in the form 

 of ammonia, over nineteen per cent of the total nitrogen 

 appearing in that form. 



In order to ascertain how far the possible presence of these 

 " hexone " bases in antipeptone may affect the physiological 

 action of the latter substance, an experiment was tried with a 

 dog of 2.5 kilos body-weight in which 25 c.c. of a solution 

 containing 0.73 gram of the mixed bases (from the phospho- 

 tungstic acid precipitate) in the form of acetates were injected 

 intravenously. The substance injected contained 15.5 per 

 cent of nitrogen when dry. As a result it was found that 

 the clotting time of the blood was not noticeably affected, 

 while arterial pressure quickly fell from 135 mm. to 50 mm. 

 Hg., but returned to the normal within three minutes. 



