330 ON ABSORPTION FROM 



fied by this procedure, as the protocols will show. The 

 colored fluid (indigo-carmine solution) was always introduced 

 into the peritoneal cavity with a blunt pipette * through a 

 small opening in the linea alba near the sternum. The ab- 

 dominal wounds were all carefully stitched afterwards. The 

 details of some experiments follow. 



I. Dog, 16 kilos. Cannula in thoracic duct and in each ureter. 

 The lymph flow in ten minutes was 2.3 c.c. ; urinary flow was 

 good. At 12.30, 20 c.c. strong indigo-carmine solution were in- 

 troduced into the peritoneal cavity. At 12.46 the urine was 

 tinged blue; the lymph continued normal in color until 12.58- 

 12.59, when a faint blue tinge was evident. The urine, at this 

 time, was perfectly dark with blue. At 1.05 the lymph showed 

 a deeper blue, but the color was not intense ; at 1.20 the color 

 began to deepen, and still persisted when the experiment was 

 concluded at 2.30. The urine continued to show a very dark 

 blue color. 



Thus the color appeared in the urine in sixteen minutes ; in 

 the lymph in twenty-eight minutes. 



II. Dog, 20 kilos. Cannula in thoracic duct and in each 

 ureter ; cannula for bleeding in femoral artery. The urine and 

 lymph flowed regularly, the latter at the rate of 1.9 c.c. in ten 

 minutes. At 12.22, 20 c.c. saturated indigo-carmine solution 

 were introduced into the peritoneal cavity. A bluish tint 

 appeared in the urine at 12.31, becoming very deep at 12.34. 

 The color began to show in the lymph at 12.45 and gradually 

 increased in intensity. At 12.47 a blood sample was drawn; 

 the serum was obtained by centrifugalization and was precipi- 

 tated with alcohol. The alcoholic filtrate was decidedly blue 

 at a time when the lymph was only beginning to show a trace 

 of color. At 2.30, when the experiment was concluded, the 

 urine was still intensely blue with pigment, while the lymph 

 was light blue. 



Thus the color appeared in the urine in nine minutes ; in the 

 lymph in twenty-three minutes. 



* Adler and Meltzer caution against the use of a sharp cannula for this 

 purpose, since it may enter the intestinal lumen occasionally: Journal of 

 Experimental Medicine, 1896, i, p. 493. 



