THE PERITONEAL CAVITY. 331 



III. Bitch, 9 kilos. Cannula in thoracic duct. Catheter in- 

 troduced exteriorly through urethra into the bladder. Lymph 

 flow in ten minutes was 1.9 c.c. The urine flowed readily when- 

 ever pressure was applied over the bladder ; otherwise the flow 

 was not regular, a few drops being expelled occasionally with 

 respiratory movements. During the experiment the urine was 

 expressed at intervals of about three minutes. At 4.01, 15 c.c. 

 strong indigo-carmine solution were introduced into the perito- 

 neal cavity. At 4.10 the urine was distinctly blue, and the color 

 had become intense at 4.20, when the first bluish tint appeared 

 in the lymph. The color deepened somewhat, but had decreased, 

 if anything, at the conclusion of the experiment at 5.05. A 

 post-mortem examination showed the blue color well distributed 

 throughout the peritoneal cavity. 



In this experiment the possibility of escape of color along wounds 

 in the abdomen was excluded. Nevertheless, the color appeared 

 in the urine in nine minutes ; in the lymph in nineteen minutes. 



IV. Bitch, 10 kilos. Cannula in thoracic duct; catheter in 

 bladder as in Exp. III. Urine was readily obtained by gentle 

 pressure. The lymph flow in ten minutes was 2 to 3 c.c. At 

 11.35 2 c.c. indigo-carmine solution were introduced into the 

 peritoneal cavity. At 11.53 the urine showed the blue color, 

 which soon deepened. No trace of blue could be detected in 

 the lymph within an hour. Accordingly, at 12.43, 10 c.c. aqueous 

 indigo-carmine solution were introduced in the peritoneal cavity. 

 At 1.0 the urine was decidedly more blue than before; the lymph 

 assumed a bluish tint (?) which became distinct at 1.05, growing 

 deeper and remaining blue when the animal was bled to death 

 at 2.15. Practically all the colored fluid had disappeared from 

 the peritoneal cavity. The diaphragm alone was stained deep 

 blue. 



Thus the color appeared in the urine in eighteen minutes, while 

 no blue could be detected in the lymph within an hour; and 

 again in the urine within seventeen minutes, and in the lymph 

 somewhat later. The experiment is interesting because of the 

 method employed to collect the urine and of the small amount 

 of indigo-carmine at first introduced. 



The following details are included from additional experi- 

 ments in which the lymph flow was accelerated by intravenous 



