342 



OBSERVATIONS ON THE NITROGENOUS 



EXPERIMENT V. 

 Three cats were employed. The pancreas fed was from calves. 



are reported by Salkowski.* These results have since repeat- 

 edly been verified in this laboratory. 



Our attention has been directed to the occurrence of 

 allantoin under similar conditions of diet in the cat. In no 

 case has this substance been missed hi the urine after thymus 

 or pancreas feeding. Thus, in one of the experiments, the 

 urine obtained after feeding 175 grams of desiccated thymus 

 (Armour's) to four cats was concentrated to a syrup and 

 extracted with alcohol. The alcoholic extracts deposited 1.6 

 grams of characteristic crystals on standing. After recrystal- 

 lization, the preparation began to melt at 215 C. and gave 

 the characteristic reaction with furfurol. An analysis of the 

 preparation gave 34.99 per cent N ; theory, 35.44 per cent N. 

 The crystals were thus identified as allantoin. Again, after 

 feeding about 2 kilos of fresh pancreas, 2 grams of allantoin 

 were separated in the form of characteristic crystals. After 

 recrystallization they began to melt at 216-217 C. and showed 

 a nitrogen content of 35.12 per cent. Salkowski f obtained 

 3.08 grams of allantoin from the urine of a small dog to which 

 If kilos of ox-pancreas were fed in five days. It is not 

 uncommon to find crystals of allantoin separating from cat's 



* Salkowski, Centralblatt fur die Medicinischen Wissenschaften, 1898, 

 p. 929. 



t Salkowski, loc. cit, p. 930. 



