FROM PROTEID SUBSTANCES. 357 



combined sulphuric acid. Finally it was washed with weak 

 and strong alcohol and lastly with ether. When dry, the 

 finely powdered substance was repeatedly boiled with distilled 

 water to insure the complete removal of any adherent soluble 

 salts. 



Analysis of a sample of this product, dried at 110 C. until 

 of constant weight, gave the following results : 



1. 0.3397 gram substance gave 0.2098 gram H 2 = 6.86 per cent H and 



0.6654 gram C0 2 = 53.45 per cent C. 



IL 0.3439 gram substance gave 0.2114 gram H 2 O = 6.83 per cent H and 

 0.6719 gram CO 2 = 63.29 per cent C. 



III. 0.2259 gram substance gave by the Kjeldahl method 0.03054 gram N = 



13.52 per cent N. 



IV. 0.2502 gram substance gave 0.03429 gram N = 13.70 per cent N. 



V. 0.6210 gram substance gave by fusion with NaOH* and KN0 8 0.1001 



gram BaSO 4 = 2.21 per cent S. 

 VI. 1.0297 grams substance gave 0.0031 gram ash = 0.30 per cent. 



PERCENTAGE COMPOSITION OF THE ASH-FREE ANTIALBUMID. 



I. II. m. IV. V. Average. 



C 63.59 63.45 ... ... ... 53.52 



H 6.86 6.83 ... ... ... 6.84 



N ... ... 13.56 13.74 . . . 13.65 



S ... ... ... ... 2.22 2.22 



... ... ... ... .. 23.77 



100.00 



Comparison of the following figures shows the relationship 

 in composition between the antialbumid and the mother sub- 

 stance from which it was derived. 



Coagulated Egg-albumin, t Antialbumid. 



C 52.18 63.52 



H 6.93 6.84 



N 15.81 13.66 



S 1.87 2.22 



O 23.21 23.77 



Most conspicuous is the marked loss of nitrogen and the 

 corresponding rise in carbon, results which accord more or 



* Pure NaOH prepared from metallic sodium and free from sulphur. The 

 fusion was made over an alcohol lamp. 



t Chittenden and Bolton, Studies in Physiological Chemistry, Yale Uni- 

 versity, 1887, ii, p. 130. 



