FROM PROTEID SUBSTANCES. 359 



I. 0.1971 gram substance gave 0.1245 gram H a O = 7.01 per cent H and 



0.3937 gram C0 2 = 54.48 per cent C. 



II. 0.3016 gram substance gave 0.6035 gram C0 2 = 54.68 per cent C. 

 in. 0.2972 gram substance gave by the Kjeldahl method 0.04113 gram N = 



13.84 per cent N. 



IV. 0.2093 gram substance gave 0.02860 gram N = 13.66 per cent N. 

 V. 0.5487 gram substance gave by fusion with NaOH and KNO 8 0.1058 gram 



BaSO 4 = 2.63 per cent S. 

 VI. 0.4770 gram substance gave 0.0015 gram ash = 0.31 per cent. 



PBBCENTAGE COMPOSITION OF THB ASH-FREE SUBSTANCE. 



L n. m. IV. V. Average. 



C 54.65 54.75 ... 54.70 



H 7.02 ... ... ... ... 7.02 



N ... ... 13.88 13.71 . . . 13.79 



S ... 2.64 2.64 



O ... ... ... ... ... 21.85 



100.00 



From these data it is evident that the antialbumid which 

 has resisted this long continued treatment with dilute sul- 

 phuric acid has gained somewhat in its content of sulphur 

 and still more in its content of carbon. Nitrogen, on the 

 contrary, remains essentially the same. This tendency of an 

 antialbumid, on hydrolysis, to grow richer in carbon usually 

 at the expense of the nitrogen is exceedingly characteristic. 

 Thus, it was found by Kiihne and Chittenden * on subjecting 

 a sample of antialbumid (from egg-albumin) to the action of 

 an alkaline solution of trypsin that the antialbumid remaining 

 undigested contained 55.54 per cent of carbon when dry, 

 while the original antialbumid contained 53.79 per cent of 

 carbon. Similarly, an antialbumid, prepared from serum- 

 albumin, containing 54.51 per cent of carbon and 14.31 per 

 cent of nitrogen, on being subjected to the action of trypsin 

 in an alkaline medium yielded an undigested residue contain- 

 ing 58.09 per cent of carbon and 12.61 per cent of nitrogen ; 

 results which clearly testify to the innate tendency of antial- 

 bumid under suitable conditions to undergo hydrolysis and 

 presumably also cleavage with formation of a more insoluble 

 and resistant body, of the antialbumid type, with a higher 

 content of carbon. 



* Kiihne and Chittenden, loc. cit 



