OF BIRDS* BLOOD. 373 



elementary analysis. The substances isolated all gave the 

 cholesterin-like reactions described for them. 



Hen serum. Several preparations of cholesteryl oleate were 

 separated from the serum of hens' blood and purified. They 

 all melted at 43-44 C. An analysis of two products gave 

 the following results: 



ANALYSIS OF CHOLESTERYL OLEATE. 



Preparation I. Preparation H. Hurthle's Average. C^H^O, : Theory. 



per cent. per cent. per cent. per cent. 



Carbon 82.68 82.49 82.84 83.02 



Hydrogen 12.04 11.90 11.77 11.95 



The approximate yield of cholesteryl oleate did not vary 

 greatly. Thus : 



I. 500 c.c. serum yielded 0.24 gram = 0.05 per cent. 

 II. 610c.c. " 0.19 " =0.03 



HI. 1660 c.c. " 0.78 " = 0.05 " 



IV. 2200 c.c. " 1.05 " = 0.05 " 



From over four liters of serum about 0.2 gram sub- 

 stance was obtained, having a melting point (after recrystal- 

 lization) of 77-78 C. and thus corresponding with the 

 cholesteryl palmitate. The crystalline form also confirmed 

 this deduction. 



Turkey serum. In one instance a relatively large yield of 

 cholesteryl oleate was obtained from turkey serum. Thus : 



I. 600 c.c. serum yielded 0.37 gram = 0.06 per cent. 

 II. 1000 c.c. " 1.43 " = 0.14 



The preparations melted at 43-44 C. and one of them (II.) 

 showed the following composition on analysis : 



Carbon 82.84 per cent. 



Hydrogen 11.79 " 



A preparation corresponding in crystalline form and solu- 

 bilities with cholesteryl palmitate melted (after recrystalliza- 

 tion) at 77-78 C. 



Goose serum. From 220 c.c. of this serum about 150 mgr. 

 (0.07 per cent) of characteristic rosettes of needles were 



