PARANUCLEIN FROM CASEIN. 397 



solved in 1 per cent sodium carbonate solution. This fluid, 

 after exact neutralization with dilute hydrochloric acid, was 

 thymolized and then dialyzed in running water until all 

 traces of chlorides were removed. The resultant neutral 

 solution of paranuclein was concentrated on the water bath 

 to a thick syrup, and while still warm was precipitated with 

 95 per cent alcohol. Upon standing for some time the pre- 

 cipitate was filtered, extracted thoroughly with alcohol and 

 ether, and dried at 105 C.* It gave the following analysis: 



Ash = 13.33%. 



Total phosphorus = 1.65%. Phosphorus found in ash = 1.73%. 



Phosphorus in substance in organic combination = 0.0%. 



Preparation B. Paranuclein was placed in water and suffi- 

 cient sodium hydroxide added to hold the substance in solu- 

 tion. By repeated nitration a perfectly clear solution was 

 obtained, and this was precipitated with acetic acid. The 

 resulting precipitate was washed by decantation with distilled 

 water, filtered, and the substance redissolved and reprecipitated 

 twice according to the method just described. After the 

 final precipitation the paranuclein was treated with alcohol 

 and ether, extracted with ether in a Soxhlet apparatus for 

 several days, and finally dried at 105 C. Analysis gave the 

 following results : 



Ash = 1.68%. 



Total phosphorus = \ % 67 f ' Phosphorus found in ash = 0.0% 

 Phosphorus in substance in organic combination = 2.75%. 



DIGESTION II. 



This digestion was carried out in a manner analogous to 

 that already described, with the exception that all treatment 

 of the resultant paranuclein with alkali as well as the sub- 

 sequent process of dialysis was avoided. The analysis of the 

 product thus prepared without further purification follows : 



* This method of procedure agrees quite closely with that described bj 

 Chittenden, loc. cit. 



