398 THE PHOSPHORUS CONTENT OF THE 



Preparation C. 



Ash = 2.91%. 



Total Phosphorus = 2.04% . Phosphorus found in ash = 0.0% . 



Phosphorus in substance in organic combination = 2.10%. 



DIGESTION III. 



In order to obtain further light on the influence which the 

 method of preparation might exert upon the analysis and 

 composition of the product, paranuclein was again prepared 

 according to the method outlined hi Digestion I. Portions 

 of the same product were treated as follows: 



Preparation D. This preparation corresponds to prepara- 

 tion A. It served as a standard for comparison with the 

 products to follow. Analysis gave: 



A , j n.80%. 



A8h = i 11.92$. 



Total phosphorus = < 2 997 Phosphorus found in ash = 2.47% . 



Phosphorus in substance in organic combination = 0.44%. 



It will be noticed that, as in the case of preparation A, the 

 ash content was very high, and in correspondence with the 

 latter nearly all of the phosphorus was recovered in the ash. 



Preparation E. This preparation received treatment simi- 

 lar to preparation c, the final digestive product, however, 

 having been simply dissolved in 1 per cent sodium carbonate 

 solution, filtered, precipitated with hydrochloric acid, again 

 filtered, washed, and prepared for analysis. 



Total phosphorus = j o 447 Phosphorus found in ash = 0.10%. 



Phosphorus in substance in organic combination = 2.36%. 



Preparation P. This preparation was treated like B with 

 the additional feature that it was subjected to dialysis after 

 repeated solution and reprecipitation by means of acid. It 

 differs from preparation E only in the fact that it was care- 

 fully neutralized before dialysis. Analysis gave the following 

 results : 



