132 Appendix. 



salides of the Grapta so exposed produced unchanged 

 Umbrosa, the co-form with Fabricti. But all chrysalides 

 from the same lot of eggs, and not exposed to cold, also 

 produced Umbrosa. Nothing was learnt, therefore, re- 

 specting this species. 



But chrysalides of Ajax, exposed at same time, did 

 give changed butterflies to some extent. From a lot of 

 8, placed in the box when under twelve hours from 

 pupation, and left for twenty-four days, there came 5 

 males and 3 females. Of these was I Telamonides in 

 markings and coloration, and all the rest were between 

 Marcellus and Telamonides. Two other chrysalides on 

 ice for twenty-three days gave Telamonides, but 3 more 

 exposed twenty-six days, and all one hour old when put 

 on ice, were unchanged, producing Marcellus. 



During the same season 6 other Ajax chrysalides were 

 placed in the box, and kept at about o 1 R. One was 

 one hour old, and remained for five days ; I was one 

 hour old, and remained for two days and three-quarters ; 

 3 at three hours old for eight days ; and I (age omitted), 

 six days. All these gave unchanged butterflies of the 

 form Marcellus. 



EXP. 2. In May, 1878, many chrysalides were placed 

 in the ice-box, being from eggs laid by Ajax, var. Walshii. 

 The youngest were but ten to fifteen minutes from pu- 

 pation, and were soft ; others at intervals up to twenty- 

 four hours (the chrysalis is hard at about twelve hours) ; 

 after that, each day up to eight days after pupation. 

 All were removed from the box on the same day, 28th 

 May. The exposure was from nineteen to five days, those 

 chrysalides which were put on ice latest having the shortest 

 exposure. The author wished to determine if possible 

 whether, in order to effect any change, it was necessary 

 that cold should be applied immediately after pupation 

 or if one or several days might intervene between pu- 

 pation and refrigeration. Inasmuch as no colour begins 



