1 34 Appendix. 



Five chrysalides lived through the winter, and all gave 

 Telamonides in the spring of 1879. 



It appeared, therefore, that the only effect produced 

 by cold in all chrysalides exposed more than three days 

 after pupation was to retard the emergence of the but- 

 terfly. But even in some of these earliest exposed, and 

 kept on the ice for full nineteen days, the only effect 

 seemed to be to retard the butterfly. 



EXP. 3. In June, 1879, eggs of the form Marcellus 

 were obtained, and in due time gave 104 chrysalides. Of 

 these one-third were placed in the ice-box at from twelve 

 to twenty-four hours after pupation, and were divided 

 into 3 lots. 



ist, 9 pupae, kept on ice 14 days. 

 2nd, 12 20 days. 



3rd, II,, 25 days. 



Temperature o 1 R. most of the time, but varying 

 somewhat as the ice melted. (Both in 1878 and 1879 

 Mr. Edwards watched the box himself, and endeavoured 

 to keep a low temperature.) 



Of the 69 chrysalides not exposed to cold, 34 gave 

 butterflies at from eleven to fourteen days after pupation, 

 and I additional male emerged nth August, or twenty- 

 two days at least past the regular period of the species. 



Of the iced chrysalides, from lot No. I emerged 4 

 females at eight days and a half to nine days and a half 

 after removal from the ice, and 5 are now alive (Nov. 18) 

 and will go over the winter. 



From lot No. 2 emerged I male and $ females at 

 eight to nine days ; another male came out at forty 

 days ; and 5 will hibernate. 



From lot No. 3 emerged 4 females at nine to twelve 

 days ; another male came out at fifty-four days ; and 6 

 were found to be dead. 



In this experiment the author wished to see as exactly 

 as possible First, in what points changes would occur. 



