Appendix. 1 5 1 



from the twenty-day pupae, this connecting band was 

 scarcely as deeply coloured and continuous as in the 

 other 3. Beyond this change on the submarginal area, 

 whereby a band is created where naturally would be 

 only the two patches, and a slight clouding of the inter- 

 vening fulvous surfaces, there was no difference of the 

 upper surface apparent between these examples of both 

 sexes, and a long series of natural ones placed beside 

 them. 



On the under side all the males were of one type, the 

 colours being very intense. There was considerably 

 more red, both dark and pale, over the whole surface, 

 than in a series of natural examples in which shades of 

 brown and a bluish hue predominate. No charge was 

 observed in the females on the under side. 



It appears that fourteen days were as effective in pro- 

 ducing changes as a longer period. In fact, the most 

 decided changes were found in the females exposed the 

 shorter period. It also appears that with this species 

 cold will produce change if applied after the chrysalis 

 has hardened. The same experiments were attempted 

 in 1878 with pupae of Grapta Comma. They were put 

 on ice at from ten minutes to six hours after forming, 

 and subjected to a temperature of about o 1 R. for 

 eighteen to twenty days, but every pupa was killed. 

 Chrysalides of Papilio Ajax in the same box, and 

 partly exposed very soon after pupation, were not 

 injured. It was for this reason that none of the 

 Interrogationis pupae were placed in the box till six 

 hours had passed. 



It appears further that cold may change the markings 

 on one part of the wing only, and in cases where it does 

 change dark or dusky markings melanises them;or it 

 may deepen the colours of the under surface (as in the 

 females of the present experiment). The females in 

 the above experiment were apparently most susceptible 



