PART I- GENERAL BACTERIOLOGY 

 CHAPTER I 



MORPHOLOGY AND ELEMENTARY 

 TECHNIQUE 



EXERCISE 1. CLEANING GLASSWARE. 



GENERAL DIRECTIONS. All glassware to contain culture media 

 must be thoroughly cleaned. New glassware should be washed in 

 hot soap-suds (a test-tube brush will be needed for the test-tubes), 

 rinsed in tap water and then placed for a few minutes in water to 

 which about \% of hydrochloric acid has been added to remove 

 free alkali frequently present on new glass, and then thoroughly 

 rinsed in running water. It is then allowed to drain. Test-tubes 

 and flasks are best dried by placing them on a drain board spe- 

 cially prepared, or standing them mouth down in a box with a 

 cloth bottom or in a wire basket. 



Glassware containing media (discarded cultures, etc.,) is best 

 cleaned by first standing in water for some hours, or by being 

 steamed and pouring out the material while in a liquid condition 

 and then cleaning as above with the exception of the use of the 

 hydrochloric acid. 



REFERENCES. A. 126 ; H. 44 ; P. 223. 



SPECIAL DIRECTIONS. Read Rule I. Clean as directed above, 

 all flasks, test-tubes, fermentation tubes and Petri dishes in your 

 possession. 



