LABORATORY RULES 



I. Before beginning an exercise read over the directions and look up 

 some of the references. Keep notes of everything done and the conclusions 

 reached on the right hand pages in this Guide. Make drawings wherever 

 they will be of value. Outline with pencil and fill in with India ink. The 

 Laboratory Guide should be kept in the laboratory. 



II. All possible cleanliness should be observed in the care of apparatus, 

 desk, etc. 



III. After working with the pathogenic bacteria the tables should be 

 washed with corrosive sublimate and the hands disinfected by washing in 

 the sublimate solution (or a germicidal soap) and then in soap and water. 



IV. Solid material, culture media and corrosive sublimate should not 

 be put in the sink but in crocks provided for the purpose. Burnt matches, 

 pieces of paper, etc., should also be put in the crocks and not on the floor. 



V. When using the steam sterilizer see that there is enough water pres- 

 ent before lighting the gas and do not leave the laboratory until the gas has 

 been turned off. 



VI. Food should not be eaten in the laboratory and lead pencils or labels 

 should not be moistened with the tongue. 



VII. All cultures of bacteria should be labeled with the name of the 

 organism, the name of the student and the date. 



VIII. The platinum needles used in making cultures should be sterilized 

 shortly before and immediately after use, and before they are laid down. 

 When the needles are covered with infectious material they should be held 

 at the side of the flame until dry before being sterilized; this will avoid the 

 danger of scattering this material about the laboratory. 



IX. Discarded cultures should be covered with corrosive sublimate and 

 placed in a proper receptacle, and under no condition should they be left 

 lying about the laboratory. Pipettes which have been used to handle infec- 

 tious material should be placed in a glass cylinder containing a disinfectant, 

 or potassium bichromate and sulphuric acid. 



X. If infectious matter should by accident come in contact with the 

 hands, or be dropped on the table or floor, corrosive sublimate (1:1000) should 

 be immediately applied. 



(XI) 



