TABLE OF CONTENTS. 



List of Apparatus X 



Laboratory Eules XI 



List of Keferences XII 



PAET I. GENERAL BACTEEIOLOGY 

 CHAPTER I. MORPHOLOGY AND ELEMENTARY TECHNIQUE. 



EXERCISE PAGE 



1. Cleaning Glassware 2 



2. Plugging Flasks and 



Tubes 4 



3. Sterilization of Glassware 4 



4. Preparation of Bouillon... 6' 



5. Filling Test-tubes and 



Flasks with Culture Me- 

 dia 10 



6. Sterilization of Culture 



Media 10 



7. Preparation of Gelatin. . . 14 



8. Preparation of Agar 16 



9. Preparation of Potatoes.. 18 



10. Preparation of Water- 



blanks 18 



11. Care of Culture Media 18 



12. Platinum Needles 20 



13. Test-tube Cultures 22 



14. Incubation of Cultures. ... 24 



15. Study of Test-tube Cul- 



tures 26 



16. Cleaning Slides and Cover- 



glasses 26 



17. Preparation of Staining 



Solution 28 



18. Simple Cover-glass Prep- 



arations.. . 30 



EXERCISE PAGE 



19. Use of Microscope 34 



20. Drawing Bacteria 36 



21. Hanging-drop Prepara- 



tion 38 



22. Microscopical Study of 



Form Types 40 



23. Study of Cell Grouping. . . 42 



24. Study of Involution Forms 46 



25. Study of Endospores 46 



26. Flagella Stain 48 



27. Capsule Stain 50 



28. Stain for Metachromatic 



Granules 50 



29. Morphology of Yeasts and 



Moulds Compared with 



Bacteria 50 



30. Gelatin Plate Cultures 52 



31. Agar Plate Cultures 56 



32. Koll Cultures 56 



33. Study of Plate Cultures... 58 



34. Use of Decolorizing 



Agents 60 



35. Gram's Stain 60 



36. Tubercle Stain (Gabbett) 62 



CHAPTER II. PHYSIOLOGY OF BACTERIA. 



EXERCISE PAGE 



37. Preparation of Special 



Media 64 



38. Effect of Keaction of Me- 



dia on Growth 66 



39. Effect of Concentration of 



Media on Growth . . 66 



EXERCISE PAGE 



40. Effect of Temperature 



Variations on Bate of 

 Growth 68 



41. Determination of Thermal 



Death Point.. 68 



(VII) 



