GENERAL BACTERIOLOGY 



83 



/. Spores : time required for formation ; media ; position in cell, 

 center or end; effect on shape of cell, clostridium, or drumstick; 

 germination, time, temperature; stain, Hauser or Moeller's method; 

 temperature limits. 



g. Vacuoles (plasmolysis). 



h. Crystals. 



i. Involution forms. 



j. Pleomorphism. 



1. Effect of various media. 



2. Effect of reaction of media. 



CULTURE CHARACTERS. 



PLATE- CULTURES (Gelatin and Agar). 



I. Surface Colonies. 



1. Form: Punctiform, too small to be denned by the naked 

 eye; circular; oval; fusiform, spindle-shaped, tapering at each end; 

 cochleate, twisted like a snail shell (Fig. 20, A) ; conglomerate, an 

 aggregate of similar colonies (Fig. 20, B) ; ameboid, very irregular 

 like the changing forms of amebae (Fig. 20, C) ; rhizoid, of an 

 irregular branched root-like character (Fig. 20, D) ; floccose, of a 

 dense woolly structure; curled, filaments in parallel strands, like 

 locks or ringlets (Fig. 20, E) ; myceloid, a filamentous colony with 

 the radiate character of a mould (Fig. 20, F) ; filamentous, an irreg- 

 ular mass of loosely woven filaments (Fig. 20, G) ; rosulate, shaped 

 like a rosette. 



FIG. 20. Types of Colonies. A. Cochleate (B. coli, abnormal form). B. Conglomerate 

 (B. Zopfii). C. Ameboid (B. Vulgatus). D. Rhizoid (B. mycoides). E. Curled (B. an- 

 tnracis). F. Myceloid (B. radiatus). G. Filamentous. 



