88 GENERAL BACTERIOLOGY 



PHYSIOLOGICAL CHARACTERS. 



a. Effect of desiccation. 



fe. Relation to temperature: minimum; optimum; maximum; 

 thermal death point. 



c. Relation to oxygen : under mica plate ; in hydrogen or nitrogen. 



d. Relation to light, Buchner's Experiment (46). 



e. Relation to antiseptics and disinfectants. 



/. Pigment production : relation of development to oxygen ; rela- 

 tion of development to character of medium ; changes produced by 

 alkali and acid ; solubility ; spectrum analysis. 



g. Gas production : rate, quantity and formula produced in dex- 

 trose, lactose, and saccharose media. 



h. Acid and alkali production: carbohydrates present; carbo- 

 hydrates absent. 



i. Relation of growth to acidity and alkalinity of medium ; growth 

 in 1, 2, 3 and 4% alkali ; growth in 1, 2, 3, 4 and 5% acid. 



j. Reduction of nitrates : to nitrites ; to ammonia. ' 



Jc. Production of sulphuretted hydrogen. 



Z. Production of indol in sugar-free bouillon. 



m. Enzyme production ; proteolytic ; diastatic. 



n. Characteristic odor. 



o. Pathogenesis : 



1. Modes of inoculation by which its pathogenic properties are 



demonstrated. 



2. Quantity of material required. 



3. Duration of the disease and its symptoms. 



4. Lesions produced and the distribution of the bacteria in 



the inoculated animals. 



5. Which animals are susceptible and which are immune. 



6. Variations in virulence and the probable causes to which 



they are due. 



7. Detection of toxic or immunizing products of growth. 



8. Agglutinating properties of serum of immune animals. 



(Widal reaction.) 



9. Lysogenic properties of serum of immune animals. 



( Pf eiff er 's phenomenon. ) 



REFERENCES. Chester, Reports Delaware Experiment Station, 

 1897, 1898 and 1899; A. 227; C. 17; H. 105; P. B. C. (Cheesman's 

 Charts) ; Kendall, Rept. Am. Pub. H. Assn., 28: 481. 



