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102 PHYSIOLOGICAL CHARACTERS 



1. RELATION TO TEMPERATURE: ,. 



optimum C.; limits to C.; 



thermal death-point C.: time of exposure minutes; 



medium in which exposure is made 



2. RELATION TO FREE OXYGEN: 



3 RELATION TO OTHER AGENTS, SUCH AS 



desiccation, light, disinfectants, etc.:. 



4. PIGMENT PRODUCTION: 



5. GAS PRODUCTION IN SUGAR MEDIA: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H : CO2: : : 



&. lactose c. saccharose 



6. ACID OR ALKALI PRODUCTION: . . . . , 



litmus milk 



7. REDUCTION OF NITRATES: 



to nitrites to ammonia... 



8. INDOL PRODUCTION 



48 hours days 



9. ENZYME PRODUCTION: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. CHARACTERISTIC ODOR: 



11. PATHOGENESIS (or other special characters) ; 



