EXERCISE 65. PHOSPHORESCENT CLASS. 121 



Bacterium phosphorescens (Cohn) Fischer. 



SYNONYMS. Photobacterium phosphorescens Beijerinck. 



EXPLANATORY. First described by Fischer in 1887. Found in Kiel harbor 

 on dead sea fish, oysters and occasionally on meat in shops. The production 

 of light is shown in the dark, especially when the organism is grown on a 

 medium made by boiling two salt herrings in a liter of water, adding 100 

 gms. of gelatin to the filtrate without neutralization, tubing and then ster- 

 ilizing (Lehmann). Phosphorescence can even be restored to attenuated 

 cultures by growth on this medium. Inasmuch as oxygen is necessary to 

 light production surface growths are best. 



EEFERENCES. Fisher, Zeitschrift fur Hygiene, 1887, Band 2, p. 92; C. 181; 

 L. &N. 231; Mig. 2: 433. 



MORPHOLOGICAL CHARAQTERS : 



SKETCHES. 



1. FORM AND ARRANGEMENT: 

 a. Bouillon 



&. Agrar. 



c. Gelatin. 



d. Other media.. 



2. SIZE: 



3. STAINING POWERS: 



a. Aqueous gentian- violet. . 



b. Loemer's methylen-blue . 



c. Gram's stain 



d. Special stains 



4. MOTILITY : 



a. Character of movement. . 

 6. Flagrella stain 



5. SPORES:. 



6. SPECIAL CHARACTERS:.... 



a. Capsules 



6. Involution forms 



c. Deposits or vacuoles.. 



d. Pleomorphism, 



