144 PHYSIOLOGICAL CHARACTERS 



1. RELATION TO TEMPERATURE : 



optimum C. ; limits to C. ; 



thermal death-point C. ; time of exposure minutes ; 



medium in which exposure is made 



2. RELATION TO FREE OXYGEN:... 



3. RELATION TO OTHER AGENTS, SUCH AS 



desiccation, light, disinfectants, etc.:. 



4. PIGMENT PRODUCTION; 



5. GAS PRODUCTION IN SUGAR MEDIA: 



a. dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



b. lactose c. saccharose 



6. ACID OR ALKALI PRODUCTION: 



litmus milk. 



7. REDUCTION OP NITRATES: 



to nitrites to ammonia. . . 



8. INDOL PRODUCTION 



48 hours days . 



9. ENZYME PRODUCTION: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic... 



10. CHARACTERISTIC ODOR: 



11. PATHOGENESIS (or other special characters) : 



