152 



PHYSIOLOGICAL CHARACTERS 



lo RELATION TO TEMPERATURE: 



optimum. C. ; limits to C.: 



thermal death-point C.; time of exposure minutes; 



medium in which exposure is made 



2. RELATION TO FREE OXYGEN: '. 



3. RELATION TO OTHER AGENTS, SUCH AS 



desiccation, light, disinfectants, etc. : 



4. PIGMENT PRODUCTION : 



5. GAS PRODUCTION IN SUGAR MEDIA: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm . 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



l. lactose c. saccharose 



6. ACID OR ALKALI PRODUCTION: 



litmus milk 



7. REDUCTION OF NITRATES: 



to nitrites to ammonia 



8. INDOL PRODUCTION 



48 hours days 



9. ENZYME PRODUCTION: 



proteoly tic 



digestion of gelatin digestion of casein 



diastatic 



10. CHARACTERISTIC ODOK: 



11. PATHOGENESIS (or other special characters):. 



