250 PHYSIOLOGICAL CHARACTERS 



1. RELATION TO TEMPERATURE: 



optimum C.; limits to C.; 



thermal death-point C.; time of exposure minute*; 



medium in which exposure is made 



2. RELATION TO FREE OXYGEN: 



3. RELATION TO OTHER AGENTS, SUCH AS... 

 desiccation, light, disinfectants, etc. 



4. PIGMENT PRODUCTION: 



5. GAS PRODUCTION IN SUGAR MEDIA: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



6. lactose c. saccharose 



6. ACID OR ALKALI PRODUCTION: , 



litmus milk, 



7. REDUCTION OF NITRATES: 



to nitrites to ammonia. . . 



8. INDOL PRODUCTION 



48 hours days . 



9. ENZYME PRODUCTION: 



proteoly tic 



digestion of gelatin digestion of casein . 



diastatic 



10. CHARACTERISTIC ODOR: 



11. PATHOGENESIS (or other special characters) : 



