280 PHYSIOLOGICAL CHARACTERS 



1. RELATION TO TEMPERATURE: 



optimum C. ; limits to C.; 



thermal death-point C. ; time of exposure .minutes; 



medium in which exposure is made 



2. RELATION TO FREE OXYGEN:... 



3. RELATION TO OTHER AGENTS, SUCH AS 



desiccation, light, disinfectants, etc.:. 



4. PIGMENT PRODUCTION: 



6. GAS PRODUCTION IN SUGAR MEDIA: 



a. dextrose (1) Shake culture: ; 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours percent. 



reaction in open arm 



gas formula, H: COz: : : 



6. lactose c. saccharose 



6. ACID OR ALKALI PRODUCTION : . . . 



litmus milk 



7. REDUCTION OF NITRATES: 



to nitrites to ammonia. . , 



8. INDOL PRODUCTION 



48 hours days , 



9. ENZYME PRODUCTION: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. CHARACTERISTIC ODOR: 



11. PATHOGENESIS (or other special characters) :, 



