300 



MEDICAL BACTERIOLOGY 



Inoculation into Lymphatic system. Fluid cultures, or suspen- 

 sions of bacteria, can be injected into the lymphatics by way of the 

 testicles, by plunging the point of the needle into the substance of 

 the testicle and injecting the desired amount of fluid. 



Inoculation into the Pleural Cavity. Where necessary the needle 

 is introduced into the pleural cavity between the ribs. It is very 

 difficult to perform this experiment without injuring the lung. 



Inoculation into the Anterior Chamber of the eye. Rarely prac- 

 ticed. The eye is treated with a few drops of cocaine (2% solution) 

 and then the needle is inserted through the cornea just in front of 

 its junction with the sclerotic, the needle passing into the anterior 

 chamber in a plane parallel to the plane of the iris. 



STERILIZATION OF INSTRUMENTS. These are best sterilized by 

 boiling in a solution of soda or borax for 15 minutes. This is ac- 

 complished in an especially designed apparatus or in an ordinary 

 enamel stew pan. In case of emergencies the instruments may be 

 dipped in benzine or alcohol and burned. This is less injurious to 

 the instruments than heating in the direct flame. 

 Use blank, p. 308 for preservation of data. 



OBSERVATION OF INOCULATED ANIMALS. After inoculation the 

 animals should be placed in separate cages, or, if placed together, 



they must be described or 

 marked so as to be easily identi- 

 fied. Fig. 36 shows a simple 

 cage made of galvanized iron 

 with soldered seams. After use 

 it is sterilized by boiling water 

 in it. The wire door is covered 

 with a cloth to prevent the too 

 rapid escape of steam. 



The inoculated animals must 

 be kept under constant observa- 



PIG. 36. Wesbrook's sterilizable, gal- 

 vanized-iron animal cage. 



tion and the following conditions noted : 

 a. Temperature. 

 ~b. Loss of weight. 



c. Peculiar position in cage. 



d. Loss of appetite. 



e. Condition of the coat or hair. 



/. Condition of the secretions of the air passages, conjunctiva 

 and kidneys ; diarrhea or hemorrhage from the bowels. 

 g. The condition of the seat of inoculation. 



