314 MEDICAL BACTERIOLOGY 



e. Mount in balsam. 

 B. Weigert' s Method. 



a. Lithium carmine (carmine 3 gms., saturated aqueous solution 

 of carbonate of lithium, 100 cc., a crystal of thymol, filt- 

 ered), 5 minutes. 

 Z>. Acid alcohol, 15 seconds. 



c. Wash in water. 



d. Transfer to slide and blot. 



e. Ehrlich 's anilin water gentian violet 3 minutes. 

 /. Blot. 



g. Place in potassium iodide and iodine solution (iodine 1 part, 



potassium iodide 2 parts, water 100 parts) 2 minutes. 

 h. Blot. 

 i. Decolorize in a mixture of anilin oil 2 parts and xylene 1 part, 



2 to 5 minutes. 

 j. Blot. 

 Jc. Mount in balsam. 



This stain can only be used with those organisms which take the 

 Gram stain, namely : Str. erysipelatos, M. pyogenes var. albus, M. 

 pyogenes var. aureus, Sar. tetragena, Bact anthracis, Bact. pneu- 

 moniae, Bact. rhusiopathiae, Bact' tuberculosis, Bact. leprae, Bact. 

 diphtheriae, Ps. aeruginosa, Bact. Welchii, B. Feseri, B. oedematis, 

 B. tetani and Streptothrix bovis. 



SPECIAL BACTERIOLOGICAL METHODS. 



Particular organisms may be stained as follows : 



Pus micrococci. Loeffler 's or Weigert 's method. 



Micrococcus gonorrhoeae. Loeffler 's method gives the best re- 

 sults. 



Sarcina tetragena. Loeffler 's or Weigert 's method. 



Bacterium anthracis. Loeffler 's or Weigert 's method. 



Bacterium pneumoniae (Pneumococcus). Weigert 's method. 



Bacterium pneumonicum (Friedlander's bacillus). The follow- 

 ing method is recommended for staining the capsules in sections 

 (M. & W.): 



a. Stain for 24 hours in the incubator in the following solution : 

 Saturated alcoholic solution of gentian violet - 50 cc. 

 Distilled water ------------ 100 cc. 



Glacial acetic acid - ........ 10 cc. 



6. Wash out in \% solution of acetic acid. 



c. Alcohol. 



