318 MEDICAL BACTERIOLOGY 



6. Wash in distilled water. 



c. Tannic acid solution (10%) y 2 to 1 minute. 



d. Counter-stain with a weak solution of eosin until sections are 



red. 



e. Wash in water until pink. 



/. Dehydrate in absolute alcohol. 



g. Clear in xylene and mount. 



Bacterium diphtheriae. Loeffler's or better Weigert's method. 

 Bacillus typhosus. 



a. Loeffler's methylen blue or carbol-fuchsin 15 min. to 24 hrs. 



b. Wash slightly in distilled water. 



c. Place in 30% solution of tannic acid for 10 to 60 min. 



d. Dehydrate rapidly in alcohol. 



e. Clear in xylene. 

 /. Examine. 



g. Mount in balsam. 



Such sections examined under a low power will be found to con- 

 tain heavily stained masses, which under a high power prove to be 

 clumps of bacilli. Not infrequently the bacilli are difficult to detect 

 in tissue from typhoid cadavers. 

 Bacillus Salmonii (hog cholera). Loeffler's method. 

 Bacterium WelcJiii ( gas bacillus) . Weigert 's and Loeffler 's methods. 

 Bacillus Feseri (symptomatic anthrax). Use Pfeiffer's stain: 



a. Dilute carbol-fuchsin % hour. 



&. Absolute alcohol slightly acidulated with acetic acid until 

 section is a reddish violet tint. 



c. Xylene and examine. 



d. Mount in balsam. 



Bacillus oedematis (malignant oedema). Pfeiffer's stain. 

 Streptothrix bovis (actinomyces). 



a. Ziehl's carbol-fuchsin, 10 minutes. 



6. Wash in distilled water. 



c. Picric acid (cons. ale. solution). 



d. Wcsh in distilled water. 



e. Wash in alcohol (50%). 



/. Dehydrate in absolute alcohol. 



g. Clear in xylene. 



h. Balsam. 



Tissue stained yellow, rays red. 



REFERENCES. M. & W. 239-286 ; N. 537. 



