342 MEDICAL BACTERIOLOGY 



Twenty grams each of agar-agar and peptone are dissolved in 

 one liter of boiling water, and the whole made alkaline by adding 4 

 cc. of a normal solution of sodium hydrate after having first brought 

 the reaction to the neutral point of litmus. 

 Then add, while hot, 



Sodium taurocholate .----.... 5 grams. 



Lactose 10 grams. 



Neutral Red (5% watery solution) 10 cc 



When the solution is complete the mass is filtered through cotton, 

 tubed, and sterilized in the steam sterilizer once for twenty-five or 

 thirty minutes. 



D. MEDIUM OF DRIGALSKI AND CONRADI.' 

 To two liters of -sugar-free broth add: 

 Peptone (Witte) - - - 10 grams 



Nutrose 10 grams ) 



e, -,. 1,1 -j -i A > these may be omitted 



Sodium chloride - - 10 grams [ 



and dissolve by the aid of heat. The mixture is brought to the boil 

 and sixty grams of agar-agar added, and the mixture kept boiling 

 until the agar is dissolved. Then the reaction of the mass is made 

 weakly alkaline to litmus by the addition of sodium hydrate (4% 

 sol.) and filtered. 



This being done, a mixture of litmus solution (6%) and lactose 

 (c. p.) 



Litmus sol. ------...... 260 cc. 



Lactose 30 grams. 



is added while both solutions are hot, and the whole boiled gently for 

 five minutes. Then add a solution of water-free sodium carbonate 

 (10%) in the proportion of 4 cc. (this may be omitted) followed by 

 20 cc. of a fresh solution of crystal violet (Griibler's) 0.1 gram in 

 100 cc. water tubed, and sterilized in the steam sterilizer for 20 

 minutes on three successive days. 



All suspected cultures should be tested with typhoid blood 

 (Widal reaction). 



The typhoid organism may be isolated from the stools during the 

 first two weeks of the disease. 



Microspira comma (Asiatic cholera). 



1. Microscopal examination of "rice-water" discharges for 

 spirilla lying parallel. 



2. Culture methods. Gelatin or agar plates should be made 

 from the rice-like flakes ; other flakes should be inoculated into flasks 



* Zeit. f . Hyg., 1902, Heft ii, p. 283. 



