356 MEDICAL BACTERIOLOGY 



The negri bodies are often found outside of the cell and can be 

 recognized by their form, color, and internal structure. 



C. Pasteur 's Method. 



a. The medulla of the suspected animal is removed under aseptic 

 precautions, as soon as possible after death. 



b. Place a piece of the medulla about the size of a pea, in 4 or 

 5 cc. of sterile bouillon and thoroughly grind up the same. 



c. Anesthetize a rabbit with ether, clip the hair from between 

 the eyes and ears and disinfect with a carbolic acid solution. 



d. Make a longitudinal incision through the skin and subcutan- 

 eous tissue along the median line, while a crucial incision is made 

 through the periosteum on one side of median line thus avoiding 

 hemorrhage from the longitudinal sinus. The periosteum is then 

 pushed back and a disc of the skull (14 inch in diameter) removed 

 with a trephine and the dura mater exposed. 



e. With a sterile hypodermic syringe introduce 2 or 3 drops of 

 the suspension of medulla beneath the dura mater, stitch the skin, 

 dry, and seal the wound with collodion. 



The rabbits apparently experience no inconvenience ; the wound 

 heals rapidly and the rabid symptoms appear in from 15 to 30 days, 

 although sometimes they may occur earlier or much later. 



EXERCISE 107. EXAMINATION OF MATERIAL FROM HUMAN 



AUTOPSIES. 



At human autopsies smears from the organs should be made on 

 cover-glasses and afterwards stained and examined. Plate-cultures 

 should also be made from the various organs. In all cases the sur- 

 face from which the material is to be obtained should first be burned 

 to avoid infection of cultures with extraneous germs. Portions of 

 the various organs should also be preserved and hardened in alcohol. 



