44 LABORATORY METHODS OF 



White Fibro-Cartilage. This variety is composed 

 of the following material: matrix, capsule, peri- 

 chondrium, cells and lacunae. The matrix has em- 

 bedded in it a few white fibers : hence the name. The 

 matrix is a homogeneous material, semisolid in con- 

 sistency, and will yield chondrin on boiling. The oval 

 shaped cells lie embedded all in the matrix, in groups 

 of two, four and eight, etc. The cells are those oval 

 bodies scattered all in the matrix. The lacunae are 

 those hardened areas found near the cells on all sides, 

 those white threads that run all in between the cells 

 and give the fibrous nature to the cartilage. The peri- 

 chondrium is the membrane that is found on the out- 

 side of the cartilage, and is composed of two layers: 

 one to protect the cartilage; the other to furnish new 

 cells in the growing cartilage. 



Hyaline Cartilage is composed of. a matrix, peri- 

 chondrium, cells and lacunae. 



Exercise No. 25. Mount a T. $. of trachea of a cat; 

 stain unth carmine. 



1. Cut the section with the microtome 20 microns 

 thick. 



2. Place the section on the slide and stain. 



3. Wash off excess O'f stain with acid alcohol. 



4. Wash in 70 per cent, alcohol; then 80 per cent, 

 and 90 per cent, 



