BACTERIOLOGY. 173 



all yoii can from the plate, and all you can from the 

 hahging-drorj culture, and all you can from the mi- 

 crtfecdpe arid the reaction oh certain media, also re- 

 action on certain animals. The technic will be dis- 

 cussed in this chapter. 



MICROSCOPIC EXAMINATION OF AN UNKNOWN. 



Make the spread of the twenty-four hour culture; 

 note the form, the size and the appearance of the pro- 

 toplasmic contents regarding its reaction to a stain ; the 

 method of. grouping. 



Has it a capsule? 



Do the bacteria stain with a watery stain? 



Do they require a mordant to stain them or not? 



How do they behave toward Gram's stain? 



Are they motile ? 



Do they form spores? 



Do they possess flagella? 



What is the best temperature for their growth? 



Growth on Media : What are the characteristics of 

 the growth? What is its relation to oxygen? What is 

 the best temperature for its growth? Observe its 

 growth on gelatine stab-culture, rate of growth, form 



