BACTERIOLOGY. 175 



and place it between the index and second finger ; take 

 an inoculating needle and sterilize it in the flame; let 

 it cool a little; then place it in the culture; after hav- 

 ing taken out the plugs as directed in the class room, 

 insert the needle in the culture tube, then transfer 

 an eye to the tube of medium .and replug both tubes. 

 Pass the culture on to the next. After having ster- 

 ilized the needle, pass it also. Incubate the medium 

 tube for twenty-four hours at room temperature after 

 having' labeled the tube as follows: name, date and 

 medium. , 



Hanging-drop Preparation. Take the hanging- 

 drop slide and make a preparation in the usual way 

 and examine the same. Note the following: shape, 

 size, motility and spore-formation. 



Dry Preparation. Make a dry preparation after the 

 usual manner, viz : Take the cover slip and clean the 

 same by the use of alcohol ; pass it three times through 

 the flame ; make a thin spread from the culture ; dry 

 in the air; pass over the flame until it is fixed, then 

 place the whole under the misroscope and examine. 



Watery-Stain Preparation. Make a spread in the 

 usual way and then apply the stain a few seconds ; and 

 wash in a glass of water; place under the microscope 

 and examine. 



