176 LABORATORY METHODS OF 



Spore Stain. Make a spread in the usual way and 

 stain for spores. 



Flagella Stain. Make the spread in the usual way 

 and stain for flagella. 



Gram's Stain. Place the spread on the cover in 

 the usual way ; apply the stain and note results. 



This is what the reagents give us in regards to the 

 diagnosis of bacteria. It is in order to take up 

 the appearance of cultures of a studied germ. Inocu- 

 late all the media that you have and note the results 

 on the same. Inoculate them in all the known ways; 

 i. e., stick, stab, smear and slant inoculations. 



Note all the changes that will occur in twenty-four 

 hours. This will help you very much in the diagnosis 

 of the germ in hand. 



Distribute the Media: Take three tubes of agar- 

 agar, and three tubes of gelatine and three of bouillon. 

 Inoculate one bouillon and two gelatine, one slant and 

 one stick in -the same way as the gelatine. Incubate 

 these for twenty-four hours. Note all the reactions 

 that occur in that time. 



QUIZ. 



What are bacteria? 

 Give their morphology. 



