BACTERIOLOGY. 177 



Where are they found? 



What are the media on which they grow best? 



How do we get rid of them? 



What is the best means of getting rid of spores ? 



How hot must the medium be to kill all known 

 spores ? 



What is the temperature to be obtained before you 

 can take out the glassware when you are sterilizing 

 it? 



How do you use the hot-air sterilizer? 



How do you lower the temperature in the hot-air 

 sterilizer ? 



Are media sterilized in the hot-air sterilizer? 



What are spores? 



What are chromatic granules ? 



What do you learn from the use of the hanging- 

 drop culture? 



How did you make the cell for the culture ? 

 Did you find any bacilli, any cocci, any spirilla? 

 How did you use the moist-sterilizer? 

 Why do you use the discontinuous method? 



What is meant by sterilizing? 

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