BACTERIOLOGY. 179 



What is the name of the rod and wire that you use 

 in inoculating the medium in your hanging-drop 

 culture ? 



How hot do you make the wire? 



What kind of heat did you use in sterilizing the 

 media? 



What kind of heat do you use in sterilizing instru- 

 ments ? 



Inoculation of Tubes. Watch the demonstrator in- 

 oculate a tube of bouillon, then do the same. Incu- 

 bate it for twenty-four hours. 



Make a stained spread of the material given you and 

 examine the same. 



Determine the class to which the germs belong, 

 whether cocci or bacilli. 



Consult the atlas for the answer to the query. 



Review the lesson you had the last period ; note the 

 likeness or unlikeness to this lesson. 



Are all bacteria pathogenic or not? 



Note their color in a growth? 



Make a stained preparation of the germs at your 

 table and examine. 



Note in your notebook the points observed. 



Determine the thermal death-point of streptococci 

 in bouillon. 



