184 LABORATORY METHODS OF 



ic acid. In the first tube put a loop full of the broken 

 up feces and take a loop full from this tube and trans- 

 fer to tube number two and so on. Next plate all the 

 tubes and number them. The carbolic acid kills all 

 the saprophytes and leaves only the bacillus typhosus 

 and its near congener, the bacillus coli. The gelatin 

 will not be liquefied. 



For Widal's reaction read McFarland. 



Serum diagnosis has been attempted. This is on 

 the principle of attenuation of germs by the presence 

 of another species. 



The Bacterium Pyocyanus was the antitoxic growth 

 used. This is on the principle of attenuation of germ 

 by the presence of another species. This as yet has 

 not come into use. 



Make a spread of bacillus anthracis. 



What is the morphology of the same? 



Are they often found in man? 



Give the approximate diameter of the germ and the 

 length in microns. 



Do you observe the morphology of the germs and 

 spores in them, 



Biology. The bacilli grow well on all the ordi- 

 nary media best grown at 35 degrees C. ; death-point, 

 60 degrees C. They withstand dry heat well, and 



