186 LABORATORY METHODS OP 



Use the colony counter and make calculation on the 



results. 



Make a note of the method you would follow in 

 making a bacteriologic diagnosis, stating the points 

 that you would observe in each step. 



Make a spread of bacillus tuberculosis and stain 

 with fuchsin and methyline blue the first, thirty min- 

 utes ; the second, one-half minute. Examine under the 

 oil lens. 



Make a spread of spirillum cholera and stain with 

 fuchsin. 



Tell how you would determine the following points 

 in the study of a certain germ. 

 First, the pathogenesis or non-pathogenesis. 

 Second, the chromogenesis. 

 Third, the motility. 

 Fourth, the morphology. 



Fifth, the reaction to stains, and Gram's method. 

 Sixth, the reaction on gelatin. 

 Seventh, the reaction on all media. 

 Eighth, the source. 



Ninth, the germicide for the germs. 

 Tenth, the thermal death-point for the germ. 

 Eleventh, the best temperature for its growth. 



