BACTERIOLOGY. 1 87 



How do you prevent your mind from letting slip 



the points observed? 



Make stained spread from the scrapings from your 

 mouth; note the morphology. 



Determine the death-point of streptococci as a 

 class, after the following manner : Put a number oi; 

 t. t. in an incubator and heat the water to 56 degrees 

 C. and let remain fifteen minutes. Inoculate a tube of 

 agar and place it in your locker until next period, and 

 note if there is a growth or not on the medium. If 

 there is, the germ or organism is not killed at the tem- 

 perature of 56 degrees C. 



Study Streptococcus' and Sarcina. Note the effects 

 on bouillon, and on agar-agar, on gelatin, and on pota- 

 to. (See Sternberg on Streptococcus.) 



See McFarland on biology of bacteria and note the 

 points of interest to you as a bacteriologist. See what 

 he says about the appearance of the colonies on the 

 different media; note the similarity between his de- 

 scription and your notes. Note the appearance of the 

 colonies on a gelatin plate. 



Note the color of the growth of bacillus prodigio- 

 sus, sarcina luteus, staphylococcus-pyogenes. 



Make a plate of gelatin and inoculate it with a drop 

 of water to see if there are any bacteria in the water. 



Study spore formation of bacteria. 



