190 LABORATORY METHODS OF 



What is the theory of immunity ? 



Express your opinion on the subject. 



Give the theory of phagocytosis. 



Demonstrate the anaerobic culture tube. 



Experiments on Animals. These are for the pur- 

 pose of demonstrating the pathogenesis of the germ. 

 Take the animal and put it in Voge's Holder, head 

 downward. Take an antiseptic solution, sterilize the 

 part selected for the injection, and at the same time 

 sterilize your syringe in hot water. Now remove the lid 

 of your Petri dish and draw the required amount of 

 growth in the syringe. (The syringe is graduated in 

 c.c. and i-io of c.c., so that you can get a definite 

 amount.) Inject the material in the animal and put 

 the animal in the cage until next period. Examine the 

 animal and see if it shows signs of disease. 



Taking the Temperature of an Animal. Take the 

 animal and place it in the Vogue, head downward ; put 

 the thermometer in the rectum,, and note degree. 



Make a spread of the material on the table and stain 

 with fuchsin. 



Bacillus Pyocyaneus is a short rod. It is three mi- 

 crons in length and has one flagellum; does not form 

 spores; frequently forms chains of four or six; 

 can exist with or without oxygen. It -stains well with 



