TECHNIQ REAGENTS AND STAINS. 



VEGETABLE SECTION. 



NO. I. METHOD FOR STAINING FRESH VEGETABLE SEC- 

 TIONS. 



(1) Apply section to slide and add rosanilin violet, 

 one to five minutes. 



(2) Wash in water to remove excess of stain. 



(3) Dry with blotting paper and add glycerine to 

 dehydrate. 



(4) Remove excess of glycerine and add glycerine 

 again to thoroughly dehydrate. 



(5) Wipe off excess of glycerine and add xylol 

 twice. 



(6) Apply to cover-glass a drop or two of xylol- 

 balsam, and, having wiped off the excess of xylol from 

 the slide, drop it gently (balsam down) upon the sec- 

 tion. Then apply general pressure with dissecting 

 needle to spread out the balsam. 



(7) Label and keep in a horizontal position until 

 the balsam is hardened. 



(195) 



