196 TECHNIC, 



Vegetable Sections. To stain paraffin or celloidin 

 sections of plant structures, the methods are practically 

 the same as those given below for animal sections. 



ANIMAL SECTIONS. 



NO. 2. CARMINE METHOD FOR FREE SECTIONS. 



1 I ) Apply section to slide and wash with thirty-five 

 per cent alcohol. 



(2) Add lithium carmine sufficient to cover section, 

 one to five minutes. 



(3) If necessary, remove excess of stain with acid 

 alcohol, five to ten seconds. 



(4) Dehydrate with increasing strength of alcohol 

 thirty-five per cent, seventy-five per cent, and ninety- 

 five per cent, and absolute. 



(5) Wipe off excess of alcohol, and when section is 

 partly dry add creosote to clear up, five to ten minutes. 



(6) Wipe off excess of creosote and mount with 

 balsam. 



(7) Centre cover-glass and apply pressure to spread 

 out the balsam. 



(8) Label and lay aside in horizontal position, 

 cover-glass up, until the balsam hardens. 



