REAGENTS AND STAINS. 197 



NO. 3. CARMINE) METHOD WITH AFFIXED PARAFFIN 

 SECTIONS. 



1 I ) Apply to the center of the slide a thin layer of 

 collodion-clove-oil mixture. 



(2) Center and attach the section, applying the heat 

 of a spirit or Bunsen flame. 



(3) Immerse in xylol two minutes and in turpen- 

 tine ten minutes to remove paraffin. Sections im- 

 mersed in turpentine alone should remain twenty min- 

 utes. 



(4) Wash with alcohol, decreasing strengths, using 

 thirty-five per cent alcohol last. 



(5) Apply lithium carmine, one to ten minutes. 



(6) Remove excess of stain with acid alcohol. 



(7) Dehydrate with alcohol, increasing strengths. 



(8) Dry and clear up with creosote, five to ten 

 minutes. 



(9) Wipe of excess of creosote and mount in bal- 

 sam. 



(10) Center cover-glass. 



( 1 1 ) Label and lay aside in horizontal position until 

 balsam hardens. 



