198 TECHNIC, 



NO. 4. CARMINE METHOD FOR AFFIXED CEUXDIDIN SEC- 

 TIONS. 



(1) Center section and affix with collodion mix- 

 ture. 



(2) Stain with lithium carmine, one to five minutes. 



(3) Remove excess of stain with acid alcohol, five 

 or ten seconds. 



(4) Apply seventy per cent alcohol. 



(5) Apply eighty per cent alcohol. 



(6) Apply ninety-five per cent alcohol a few sec- 

 onds. 



(7) Clear up with creosote. 



(8) Remove excess of creosote with blotting paper. 



(9) Mount with balsam and center cover-glass. 



(10) Label and lay aside in a horizontal position. 



NO. 5. HAEMATOXYUN METHOD -FOR FREE SECTIONS. 



1 I ) With section on slide, apply alcohol of the same 

 strength as staining solution. 



(2) Stain with diluted hsematoxylin, one to ten 

 minutes. 



