REAGENTS AND STAINS. 199 



(3) Remove excess of stain with thirty-five per 

 cent alcohol. 



(4) Dehydrate with alcohols, increasing strengths. 



(5) Clear up with creosote or cedar oil. 



(6) Center section and apply balsam and cover- 

 glass. 



(7) Center cover-glass. 



(8) Label and lay aside in horizontal position until 

 balsam hardens. 



NO. 6. HAEMATOXYUN METHOD FOR AFFIXED SECTIONS. 



(1) Apply to slide a thin layer of egg-albumen and 



glycerine. 



(2) Center section and flatten it by gently touching 

 with end of finger. 



(3) Apply heat of flame until paraffin melts (sec- 

 tions that have been flattened upon water should be 

 heated much longer than others). 



(4) Remove paraffin with xylol or turpentine. 



(5) Remove xylol, etc., with alcohol, decreasing 

 strengths. 



(6) Stain with diluted hsematoxylin, one to ten 

 minutes. 



